Routinely thin layer chromatography (TLC) was used for such purposes and is now replaced by faster technologies with better resolution. In principle most of the analytical techniques can be used to analyze lipids including fatty acids, phospholipids, sphingolipids, triglycerides and steroids. Picture (CC-by) thanks to: Sud M, Fahy E, Cotter D, Brown A, Dennis EA, Glass CK, Merrill AH, Murphy RC, Raetz CRH, Russell DW: LMSD: LIPID MAPS structure database. For better classification 81 general lipid classes and 276 sub-categories were generated as a nomenclature by the LipidMaps consortium. Lipids are a distinct and diverse set of small molecules consisting of eight general compound classes including (i) fatty acyls, (ii) glycerolipids, (ii) glycerophospholipids, (iv) sphingolipids, (v) sterol lipids, (vi) prenol lipids, (vii) saccharolipids and (viii) polyketides. Lipids, lipid classes, lipid-Omics and the lipid-Ome:
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